Tissue from a clear cell adenoma of the adrenal gland was incubated simultaneously with [7α-3H]pregnenolone sulphate and [4-14C]pregnenolone in Krebs—Ringer bicarbonate-glucose solution. Evidence was obtained for the conversion of pregnenolone sulphate to cortisol and corticosterone. The differences in the ability of the tissue to convert the sulphate and the free steroid to corticosteroids and androgens are discussed in relation to theories on the functional zonation of the human adrenal cortex.
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