This communication deals with the isolation, identification and quantitative estimation of 21-deoxycortisol and four other minor steroids, 11-deoxycortisol, 11β-and 17α-OH-progesterone and 11β-OH-androstenedione, in a pooled sample of adrenal venous blood from two normal possums. Adrenal venous collections were made during an i.v. infusion of porcine corticotrophin at a constant rate of 2·0 i.u./kg. body wt./hr. for periods of 2 hr. in each animal. The procedures for collecting adrenal venous blood and steroid assays have been described previously (Weiss & McDonald, 1965, 1967). Identification of the five components was based on the following criteria: chromatographic mobilities compared with reference standards in at least three of the following solvent systems, T/75, L/80, LT11/75, LT 32/80, LB 53/80 (Bush, 1961); mobility after acetylation with pyridine:acetic anhydride (2:1); u.v. absorption in methanol; reaction of the isolated steroids with blue tetrazolium, Porter-Silber and Zimmermann reagents and fluorescence in u.v. light after treatment with
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