A radioimmunoassay method for plasma corticotrophin (ACTH) is described. ACTH is extracted from plasma by adsorption to silicic acid powder and subsequent elution by an acetone—acetic acid mixture. ACTH antiserum and 131I-labelled ACTH are added and the antigen-antibody reaction proceeds for 3 days. Activated charcoal coated with plasma is used to separate antibody-bound and unbound ACTH. Factors affecting the accuracy, sensitivity, and specificity of the assay have been assessed.
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Online ISSN: 1479-6805
Print ISSN: 0022-0795
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