In experiments on the pubescent ram and bull (Skinner & Rowson, 1968) it has been shown that when one testis is made cryptorchid, or vasectomy is carried out unilaterally, the ampulla on the treated side will be smaller and its content of fructose and citric acid lower than on the non-treated, i.e. contralateral, side. The seminal vesicles and bulbo-urethral glands, however, remain unaffected by this treatment. The difference between the ampullae was attributed to a local effect of testosterone acting from within the lumen, on the treated but not on the non-treated side of the tract (Skinner & Rowson, 1968). The object of the present experiments was to test this hypothesis by injecting small quantities of testosterone down the vas deferens on one side only, in surgically castrated rams. A preliminary report has already appeared (Skinner & Rowson, 1967).
Ten lambs were castrated bilaterally at 12 weeks of age when
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