A sensitive method for the assay of calcitonin is described with a much lower detection limit than that reported hitherto. Calcitonin was injected intravenously into fasting Wistar rats and aortic blood was collected 30 min. later under anaesthesia. Equivalent hypocalcaemia was produced in 3-week-old rats by doses less than a third of those required in 5-week-old ones. Less than 0·1 m-u. calcitonin/3-week-old rat could be measured with an index of precision of about 0·2.
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