The description of the myotrophic activity assay in the rat using the levator ani muscle (Eisenberg & Gordon, 1950) has been followed by many modifications. These changes include the reduction in assay time (Hershberger, Shipley & Meyer, 1953; Metcalf & Broich, 1961; Potts, Arnold & Beyler, 1960; Sala & Baldratti, 1963), a suggested change in muscle nomenclature (Hayes, 1965) and varying statistical interpretations of the assay (Ahrén, Arvill & Hjalmarson, 1962; Ercoli, Bruni, Falconi, Gardi & Meli, 1960; Hershberger et al. 1953). Although progress has been made in these areas of the assay, a more rapid method of extirpation would be beneficial. This report describes a simple method of approaching the levator ani from the dorsal aspect of the rectum thus minimizing dissection time.
The animal is placed on its back upon a cork board and the hind paws are pinned down as shown in Fig. 1 a. A third
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