20α-Hydroxypregn-4-en-3-one (20α-dihydroprogesterone) has been isolated from porcine ovaries under in vitro conditions (Bjersing & Carstensen, 1967; Cook, Kaltenbach, Norton & Nalbandov, 1967; Schomberg, 1967). No 20β-epimer has hitherto been demonstrated in the pig.
While the progesterone concentration was being investigated in the corpora lutea of the pregnant gilt using a method similar to that of Rowlands & Short (1959), two u.v. absorbing substances were found on the paper chromatograms with Rf values identical to authentic 20α- and 20β-hydroxypregn-4-en-3-one (20α-ol and 20β-ol) These substances were eluted, and gave the same response as the authentic steroids when examined by spectrophotometry, namely an absorption maximum in ethanol at 240 mμ, an absorption maximum at 285 mμ on heating with cone. H2SO4, and 20α-ol gave an absorption maximum at 485 mμ when treated with sulphuric acid:80% ethanol (Wiest, 1959). Both substances were acetylated with 0·1 ml. pyridine and acetic anhydride
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