The biosynthetic activity of several bovine tissues was compared in slice incubation experiments based on the incorporation of 3H-labelled amino acids. An approximate parallelism was observed between general protein and peptide synthesis in each case. The highest activity was observed in hypothalamus and median eminence, followed by adeno- and neurohypophysis, while liver cells were least potent in these processes. A radioactive polypeptide preparation, isolated from hypothalamus and median eminence experiments, was found to resemble adrenocorticotrophin in certain physico—chemical properties, including molecular size and pattern of isotopic leucine incorporation.
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