OESTRIOL IN AMNIOTIC FLUID: A GAS CHROMATOGRAPHIC METHOD

in Journal of Endocrinology
Authors:
J. S. BIGGS
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A. KLOPPER
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G. R. WILSON
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SUMMARY

A method is described for estimating oestriol in amniotic fluid. The main steps of the method are acid hydrolysis, chemical purification and methylation, chromatography on alumina columns, acetylation and estimation by gas chromatography. A radioactive internal standard is employed to correct for losses during assay. Data on the reliability of the method are given.

 

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