Since Baulieu (1962) suggested that dehydroepiandrosterone sulphate (DHAS) was a natural adrenal secretory product, several endocrine tissues have been shown to form steroid sulphates. These can be metabolized and still preserve their conjugated form (Calvin & Lieberman, 1964). This report presents evidence for such transformations by polycystic ovarian tissue.
Homogenized tissue, in 3 ml. Krebs—Ringer improved bicarbonate buffer, was incubated with 25 μc [7α-3H]DHAS (Radiochemical Centre, Amersham, 605 mc/m-mole) for 2 hr. at 37°, in 95%O25%CO2. Neutral, phenolic and conjugate fractions were obtained after adding carrier steroids to the incubation mixture (Fahmy, Griffiths, Turnbull & Symington, 1968). Complete extraction of free steroids was made before conjugate extraction.
The neutral fraction (45% of recovered radioactivity) was chromatographed on
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