Plasma was obtained from adult Wistar rats maintained under 14 h of light and 10 h of darkness and shown by daily vaginal smears to have had two or more cycles of 4 days' duration. Oestradiol-17β concentrations were measured by a micro-modification (Exley, 1969) of the competitive protein binding technique of Corker & Exley (1970). In more than 75% of cases plasma from two rats was pooled (5–7 ml) and duplicate determinations carried out. At concentrations above 10 pg/ml the average variation between duplicates was ±15% of the mean. Below this level, although the mean detectable amount was 6 pg (corresponding to a concentration of about 2 pg/ml) precision was such (average agreement between duplicates ±35%) that values in this range must be regarded as estimates only. Plasma luteinizing hormone (LH) in samples from individual rats was determined by a modification (Naftolin & Corker, 1970) of the radioimmunoassay method of
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