48-hour access to this article
USD $30.00
USD $30.00
EFFECTS OF HUMAN PITUITARY EXTRACTS ON ANDROGEN BIOSYNTHESIS BY HUMAN ADRENALS IN VITRO
in Journal of EndocrinologySUMMARY
The effects of human pituitary extracts upon steroid biosynthesis by pieces of human adrenal tissue incubated in Krebs—Ringer bicarbonate buffer have been studied. Experiments with both adult and foetal adrenal tissue have been carried out. Radioactive precursor (either [7-3H]pregnenolone or [4-14C] progesterone) was added to each incubation vessel and the synthesis of radioactively labelled products was estimated by reverse isotope dilution. Individual steroids were characterized by paper and thin-layer chromatography; the radiochemical purity of cortisol, androstenedione, dehydroepiandrostenedione (DHA) and DHA sulphate was established following derivative formation. There was no consistent effect of the pituitary preparations upon the biosynthesis of cortisol, DHA or DHA sulphate, nor upon the ratio of cortisol to the combined production of DHA and DHA sulphate. However, the production of androstenedione from both [7-3H]pregnenolone and [4-14C]progesterone was significantly increased in the presence of pituitary and gonadotrophic preparations.
Journal of Endocrinology is committed to supporting researchers in demonstrating the impact of their articles published in the journal.
The two types of article metrics we measure are (i) more traditional full-text views and pdf downloads, and (ii) Altmetric data, which shows the wider impact of articles in a range of non-traditional sources, such as social media.
More information is on the Reasons to publish page.
| Sept 2018 onwards | Past Year | Past 30 Days | |
|---|---|---|---|
| Full Text Views | 0 | 0 | 0 |
| PDF Downloads | 0 | 0 | 0 |
Online ISSN: 1479-6805
Print ISSN: 0022-0795
Strengthening biomedical communities
to advance science and health
CONTACT US
Bioscientifica Ltd | Starling House | 1600 Bristol Parkway North | Bristol BS34 8YU | UK
Bioscientifica Ltd | Registered in England no 3190519
Powered by PubFactory