Freeze-etched preparations of rabbit neurohypophysis and isolated hormone granules from cow neurohypophyses were examined electron microscopically. Many features seen in conventionally prepared sections were also apparent in freeze-etched replicas, particularly the neurosecretory nerve-endings containing elementary granules and elongated pituicyte processes.
Measurements of diameters of both isolated and hormone-containing granules in situ indicated that they were slightly larger in freeze-etched material than in that prepared by conventional electron microscopic techniques.
Fractures through the cores of hormone-containing granules showed a lamellar internal structure of 4 nm periodicity in both species examined.
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Online ISSN: 1479-6805
Print ISSN: 0022-0795
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