Porcine parathyroid hormone has been isolated and purified. Its size and charge properties are similar to those of bovine and human parathyroid hormones and in a bioassay system the response to porcine hormone is parallel to that of bovine parathyroid hormone. Porcine parathyroid hormone has been labelled with 125I. The immunological properties of the porcine hormone were compared with human and bovine parathyroid hormone in a radioimmunoassay system using antisera against bovine parathyroid hormone. When labelled bovine parathyroid hormone was used as a tracer in the radioimmunoassay, porcine parathyroid hormone could be shown to differ immunologically from the hormones of the other two species. When labelled porcine hormone was used as tracer, it was displaced equally well by hormone from all three species, so that in addition to regions of difference in the molecule there are probably regions of similarity.
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