THE BINDING OF CORTISOL BY PROTEINS IN OVINE LYMPH

in Journal of Endocrinology
Authors:
J. Y. F. PATERSON
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F. HILLS
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The concentrations of cortisol-binding proteins in pre-scapular lymph of sheep were determined. Albumin was measured by the method of Watson & Nankiville (1964), based on the solubility of albumin in 1% (w/v) trichloroacetic acid in propan2-ol: water (93:7, v/v) and measurement of the extinction at 220 nm. The ratio of albumin-bound to unbound cortisol was taken to be 0·275A, where A is albumin concentration (g/100 ml) (Paterson & Hills, 1967).

Transcortin concentration, expressed as cortisol-binding capacity, was measured by the method of Doe, Fernandez & Seal (1964): portions of lymph (2 ml) containing 0·5 μCi (0·5 μg) tritium-labelled cortisol were incubated for 1 h at 37 °C, and after cooling rapidly to 4 °C were filtered through Sephadex G 50 gel at the same temperature. Transcortin was also measured by the equilibrium dialysis method of Paterson & Hills (1967): diluted lymph (1:2, v/v) was dialysed against saline solutions of tritium-labelled cortisol

 

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