The determination of the androgen excretion in the urine has, in spite of the disadvantages, been the method of choice in studies of the androgen production in the organism.
The presence of a specific acid phosphatase in prostatic tissue [Kutscher & Wolbergs, 1935] suggested that a relation might exist between the androgen production and the amount of phosphatase in semen. Gutman & Gutman , by injection of testosterone propionate in prepubertal rhesus monkeys, produced a several-fold increase of phosphatase.
The amount of phosphatase in the semen of normal men varies greatly, but in each individual is relatively constant [Gutman & Gutman, 1940]. In unpublished investigations Sury found an average of 4400 phosphatase units per ml. in 50 normal individuals, and Andersson an average of 3700 units per ml. in 173 normal individuals. In aspermia the two investigators found 3300 and 3400 units respectively. The method employed for determining
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