A pituitary was obtained from a patient with metastatic breast cancer and cultured for 81 days in Medium 199. The medium was changed every 3 days. The prolactin activity of the medium, as estimated by the local crop gland proliferation assay, was significantly lower in the first 3-day culture period than in subsequent periods. Prolactin activity was similar between days 6 and 21 of culture, decreasing to the limits of the bioassay system on the 27th day of culture. Analytical disc electrophoresis of the medium revealed the presence of a protein component which migrated behind human growth hormone (HGH). A densitometric analysis of the bands in disc electrophoresis showed that the protein concentration of the component migrating behind HGH correlated with the prolactin activity of the medium for later periods of culture. A calculation of the specific activity of this component gave a value of 125 i.u. prolactin/mg protein.
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