Diamond, Rust & Westphal (1969) originally showed that pregnant guinea-pig plasma binds progesterone about 100 times more firmly than cortisol. Work along this line yielded a competitive protein-binding method for the assay of progesterone in plasma during pregnancy.
Plasma was obtained from pregnant Casia guinea-pigs in the latter part of gesta-tion. [1-2-3H]Progesterone (6 ng, 33 Ci/mmol) in ethanol was evaporated to dryness and dissolved in 30 ml 0·04 m-phosphate buffer, pH 7·4. Fifteen microlitres guineapig plasma were added and mixed gently for 1 h at room temperature. To 100 μl plasma, 200 μl methanol were added and the mixture kept at − 20 °C for 10 min. After centrifugation an appropriate volume of the supernatant was transferred to a small glass test tube and evaporated to dryness. Benzene (100 μl) and heptafluorobutyric anhydride (3 μl) were added to the residue and the sample was kept at 62 °C for 30
Journal of Endocrinology is committed to supporting researchers in demonstrating the impact of their articles published in the journal.
The two types of article metrics we measure are (i) more traditional full-text views and pdf downloads, and (ii) Altmetric data, which shows the wider impact of articles in a range of non-traditional sources, such as social media.
More information is on the Reasons to publish page.
| Sept 2018 onwards | Past Year | Past 30 Days | |
|---|---|---|---|
| Full Text Views | 4 | 0 | 0 |
| PDF Downloads | 2 | 0 | 0 |
Online ISSN: 1479-6805
Print ISSN: 0022-0795
Strengthening biomedical communities
to advance science and health
CONTACT US
Bioscientifica Ltd | Starling House | 1600 Bristol Parkway North | Bristol BS34 8YU | UK
Bioscientifica Ltd | Registered in England no 3190519
