In a recent report (Watson, 1971) a method for the bioassay of luteinizing hormone (LH) depending on progesterone synthesis by superovulated rat ovarian tissue in vitro was described. The method, although specific and precise is, however, only useful for the determination of physiological levels of LH in small volumes of body fluids towards its lower limit of sensitivity. A method of improving the sensitivity five times by pretreatment of the experimental animals with the pituitary hormone prolactin is reported here. It has been known for some time that prolactin has the effect of inhibiting the appearance of the enzyme 20α-hydroxysteroid dehydrogenase in the rat (Wiest, Kidwell & Balogh, 1968). Thus, in incubated ovaries of prolactintreated rats, progesterone, and not its reduced form 20α-hydroxy-pregn-4-en-3-one, is the principle steroid accumulated (Armstrong, Miller & Knudsen, 1969).
The experimental procedures used were those previously described (Watson, 1971) except that in addition to priming with
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