DETERMINATION OF PLASMA OESTRIOL IN NORMAL PREGNANCY AND LABOUR USING GAS-LIQUID CHROMATOGRAPHY

in Journal of Endocrinology
Authors:
W. COOPER
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M. G. COYLE
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J. A. MILLS
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SUMMARY

A method is described for estimating oestriol in 2–10 ml samples of human pregnancy peripheral plasma. It incorporates acid hydrolysis, chemical purification, methylation, chromatography on alumina columns, formation of a derivative and quantitative determination by gas chromatography. A radioactive internal standard was added to correct for procedural losses. Plasma oestriol determinations in five normal patients throughout pregnancy and delivery are reported.

 

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