BIOASSAY OF OXYTOCIN BY CHANGES IN LIGHT PENETRATION THROUGH RAT MAMMARY GLAND TISSUE IN VITRO

J. BÍLEK; I. MIKULÁŠ; J. SLABA; I. SKÁLA

doi:10.1677/joe.0.0510771

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BIOASSAY OF OXYTOCIN BY CHANGES IN LIGHT PENETRATION THROUGH RAT MAMMARY GLAND TISSUE IN VITRO

in Journal of Endocrinology

Authors: J. BÍLEK, I. MIKULÁŠ, J. SLABA, and I. SKÁLA

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DOI:: https://doi.org/10.1677/joe.0.0510771

Page(s):: 771–NP

Volume/Issue:: Volume 51: Issue 4

Article Type:: Research Article

Online Publication Date:: Dec 1971

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SUMMARY

A method for the bioassay of oxytocin in the lactating rat mammary gland in vitro is described. The changes in transparency induced by oxytocin in a flat segment of the gland are recorded by means of a CdS photoresistor and a simple Wheatstone bridge. The sensitivity of the assay in routine use is about 0·5–10 μu. oxytocin, the index of precision being about 0·1.