Short-term experiments (Seiki, Higashida, Imanishi, Miyamoto, Kitagawa & Kotani, 1968; Seiki, Miyamoto, Yamashita & Kotani, 1969) have failed to show any specific progesterone-binding receptors in the hypothalamus and pituitary gland. These sites do not show selective uptake of progesterone compared with other parts of the brain after i.v. administration of [3H]progesterone to rats in various experimental conditions. To investigate more extensively the existence of specific receptor sites in the hypothalamus and pituitary gland, a long-term experiment was carried out.
Female Wistar rats, weighing about 230 g, were used. Ovariectomy was carried out only on animals in dioestrus, as determined by vaginal smears. Two to three weeks after the operation, the animals were given an i.v. injection of 50 μCi [7α-3H]progesterone/100 g body weight (sp. act. 18·1 Ci/μmol) in 0·2 ml 0·9% NaCl solution containing 5% (v/v) ethanol. The animals were killed by decapitation at varying intervals of
Journal of Endocrinology is committed to supporting researchers in demonstrating the impact of their articles published in the journal.
The two types of article metrics we measure are (i) more traditional full-text views and pdf downloads, and (ii) Altmetric data, which shows the wider impact of articles in a range of non-traditional sources, such as social media.
More information is on the Reasons to publish page.
| Sept 2018 onwards | Past Year | Past 30 Days | |
|---|---|---|---|
| Full Text Views | 12 | 1 | 0 |
| PDF Downloads | 2 | 1 | 0 |
Online ISSN: 1479-6805
Print ISSN: 0022-0795
Strengthening biomedical communities
to advance science and health
CONTACT US
Bioscientifica Ltd | Starling House | 1600 Bristol Parkway North | Bristol BS34 8YU | UK
Bioscientifica Ltd | Registered in England no 3190519
