The development of trophic hormones in foetal pituitary cells has been shown by the use of fluorescent antibodies, differential staining techniques and bioassay of pituitary homogenates. In foetal rat pituitaries, growth hormone, thyrotrophin and adrenocorticotrophin have been detected by radioimmunoassay and bioassay (Contopoulos & Simpson, 1957; Phillips & Schmidt, 1958; Milkovic & Milkovic, 1962; Birge, Peake, Mariz & Daughaday, 1967); but prolactin has not been detected by these techniques.
During experiments involving disc electrophoresis of rat pituitary homogenates the pituitaries from foetal rats were examined and a protein band appeared in the gel in a position almost identical with that of prolactin in the maternal pituitary (Plate). Rat pituitary hormones separated by disc electrophoresis according to the method of Davis (1964) have been identified by Jones, Fisher, Lewis & Vanderlaan (1965). Prolactin is the hormone that has the greatest mobility in this system; maternal prolactin had an RF value
Journal of Endocrinology is committed to supporting researchers in demonstrating the impact of their articles published in the journal.
The two types of article metrics we measure are (i) more traditional full-text views and pdf downloads, and (ii) Altmetric data, which shows the wider impact of articles in a range of non-traditional sources, such as social media.
More information is on the Reasons to publish page.
| Sept 2018 onwards | Past Year | Past 30 Days | |
|---|---|---|---|
| Full Text Views | 0 | 0 | 0 |
| PDF Downloads | 2 | 0 | 0 |
Online ISSN: 1479-6805
Print ISSN: 0022-0795
Strengthening biomedical communities
to advance science and health
CONTACT US
Bioscientifica Ltd | Starling House | 1600 Bristol Parkway North | Bristol BS34 8YU | UK
Bioscientifica Ltd | Registered in England no 3190519
