Rabbit antisera to ovine follicle-stimulating (FSH) and luteinizing (LH) hormones were used in the indirect fluorescent antibody technique on sections of rat testis to detect intravenously injected FSH or LH. A direct method using ferritin-labelled rabbit anti-LH globulins was also performed on sections of testis from LH-injected rats. Follicle-stimulating hormone was localized in the Sertoli cells while LH appeared mainly in the interstitial and peritubular cells. These results agree with previous observations on the cellular distribution of both gonadotrophins in the rat testis.
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