Follicle-stimulating hormone (FSH) increases the amount of [3H]thymidine taken up by cultured mouse ovaries; the response varies with hormone concentration (Ryle, 1971a). Certain derivatives of FSH, ineffective in vivo, retain activity in vitro (Ryle, Chaplin, Gray & Kennedy, 1970; Kennedy, Butt, Robinson & Ryle, 1972). The thymidine uptake procedure has been improved and simplified for measuring quantitatively the residual activity in derivatives of highly purified FSH. The following procedure is now employed routinely in studies on the relationship between molecular structure and biological function. The source and preparation of the tissue, the general culture method, the [Me-3H]thymidine, the dissolution of the tissue and the counting procedure are as described by Ryle (1971a). The Glasgow Modification of Eagle's Minimum Essential Medium (MEM) is used (Macpherson & Stoker, 1962; Biocult Laboratories Ltd., Glasgow; Flow Laboratories Ltd., Ayrshire), buffered with 20 mm-HEPES (N-2-hydroxyethylpiperazine-N'-ethanesulphonic
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Online ISSN: 1479-6805
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