A simplified assay for somatomedin (sulphation factor; Daughaday, Hall, Raben, Salmon, Van den Brande & Van Wyk, 1972) is described in which the most time consuming steps have been eliminated without loss of precision or reliability.
Twenty-two- to twenty-seven-day-old intact male Sprague-Dawley rats are starved for 48 h, killed and the costal cartilages removed, discarding the distal 6 mm of each segment (Yde, 1968). 2 mm lengths of cartilage are added in duplicate to 10 ml stoppered tubes containing 1·0 ml of a modified Eagle's medium (without phenol red and streptomycin, and containing 0·6 mM-glutamine, 2·5 mg glucose/ml, 25 units neomycin sulphate/ml, 100 units penicillin/ml, 1·0 mm-Na2SO4 and 0·05 m-tris-HCl, pH 7·4). The tubes are pre-incubated at 37 °C for 24 h, 20 μCi carrier free Na235SO4 and 0·2 ml of sample are added and the incubation continued for
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