Wilhelmi preparations of bovine growth hormone (GH) have been resolved on DEAE-cellulose into three protein fractions (Manchester & Wallis, 1963). Two of these were shown to possess growth-promoting activity. Fraction A, containing the major electrophoretic component of the original preparation - a slow moving band in starch-gel at pH 8·8 — and a trace of faster components, had growth-promoting activity about equal to that of the unfractionated material. Fraction B, comprising two or three of these faster components, varied in activity from the level of Fraction A down to 50%. This report describes the fat mobilizing activity of the two fractions in vivo and in vitro.
Hooded Norway rats weighing about 150 g were used after an overnight fast. Fat mobilizing activity in vivo was assayed by i.p. injection into female rats of preparations dissolved in 0·9% NaCl solution at pH 9·5. Assays consisted of three groups, each of six animals,
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