The rate of disappearance of luteinizing hormone (LH) from the circulation is an important parameter in studying the dynamics of the hormone and in interpreting the significance of both circulating and pituitary hormonal levels. This rate is most frequently expressed in terms of the half-life of LH in the circulation (see Parlow, 1968). The estimation of the half-life of circulating LH in birds has been made feasible by the availability of purified chicken gonadotrophins (Stockell Hartree & Cunningham, 1969; Scanes & Follett, 1972), together with a radioimmunoassay for avian LH (Follett, Scanes & Cunningham, 1972).
The half-life of LH in the domestic fowl was determined by following the dis-appearance of either unlabelled LH or 125I-labelled LH from the circulation of the bird. In the case of the labelled hormone, a chicken LH fraction of high ovarian ascorbic acid depleting activity and low thyrotrophin content (fraction AEI, Scanes & Follett,
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