The influence of exogenous gonadotrophic hormones (GTH) on the rate of spermatogenesis and germinal maturation in the testes of hypophysectomized male mice was measured. Liquid emulsion autoradiographic techniques were used to measure the incorporation of tritiated thymidine into germinal DNA to see if exogenous hormones could alter the rate of development of late spermiogenic cell stages (Gude, 1968; Clermont & Trott, 1969).
Two experiments were carried out using hypophysectomized and intact 70-day-old CD-I Charles River albino male mice (10/group). In experiment 1, intact (1A) and hypophysectomized (1B) males were compared with hypophysectomized males injected s.c. on alternate days either with 25 i.u. pregnant mare serum gonadotrophin (PMSG) (Equinex, Ayerst) (group 1C), or with 25 i.u. PMSG plus 25 i.u. human chorionic gonadotrophin (Ayerst) (group 1D). After 2 weeks of hormonal replacement, spermatogenic cells in the S phase (spermatogonia and primary spermatocytes synthesizing DNA) were labelled in vivo by a single
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