METABOLISM OF 3β-HYDROXY-5-ENE STEROIDS BY TESTES OF RATS ACCLIMATIZED TO A HOT ENVIRONMENT

in Journal of Endocrinology
Authors:
E. BEDRAK
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V. SAMOILOFF
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U. A. SOD-MORIAH
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SUMMARY

The metabolism of 3β-hydroxysteroids, in the presence of NADPH, by testicular homogenates of adult rats acclimatized to a hot environment (33–35 °C, 25–40% relative humidity), was compared with that of control and surgically produced cryptorchid testes. Prolonged exposure to a hot environment stimulated the transformation of 3β-hydroxysteroids to 3-oxo-4-ene metabolites, so that relatively large amounts of the latter accumulated. Pregnenolone was metabolized rapidly to progesterone, 17α-hydroxyprogesterone, androstenedione and testosterone. A similar trend was observed in the metabolism of 17α-hydroxypregnenolone. In-vitro synthesis of testosterone in the rat apparently takes place primarily by the 4-ene pathway. The 5-ene-17,20-lyase reaction appears to be rate-limiting. Heat acclimatization does not seem to affect this step. It does, however, seem to enhance the conversion of dehydroepiandrosterone via 5-androstene-3β,17β-diol to testosterone. Enzymic activity was much lower in cryptorchid than in heat-acclimatized testes, where it actually increased, except for 17β-hydroxysteroid oxidoreductase.

Continuous exposure of rats to a high ambient temperature for 4–6 months tended to decrease testosterone concentration in peripheral blood, and retarded growth rate and gonadal size. Histological examination showed atrophied areas in the testes where necrobiosis of the germinal epithelium had occurred. These necrotic foci, unlike those found in cryptorchid testes, were randomly scattered among intact seminiferous tubules in which active spermatogenesis was taking place.

 

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