ISOLATION AND CHARACTERIZATION OF A PITUITARY PEPTIDASE SPECIFICALLY RELEASING, FROM GROWTH HORMONE, A PEPTIDE (SOMANTIN) WITH ANTAGONISTIC ACTIVITY TO INSULIN IN VITRO

in Journal of Endocrinology
Authors:
C. J. DRIVER
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J. McD. ARMSTRONG
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J. BORNSTEIN
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F. M. NG
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SUMMARY

A peptidase has been isolated from ovine pituitary glands and has been purified. It specifically hydrolyses ovine growth hormone, releasing a peptide with somantin activity. It is proposed to call this peptidase somantin-releasing enzyme (SRE). It is a di-isopropylphosphorofluoridate-sensitive peptidase with a molecular weight of 23000 Daltons. It is optimally active near pH 8·0. It is of quite limited specificity, as it hydrolysed growth hormone to a limited extent only and did not hydrolyse most of the other proteins tested as substrates.

 

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