Changes in the metabolism of pyrimidine 5′-nucleotides and RNA in the mouse uterus during the oil-induced decidual cell reaction were investigated. During the first 20 h after the intraluminal injection of 10 μl sesame oil the amounts of HClO4-soluble nucleotides and uridine 5′-nucleotides in the treated horn increased by 55% and 115%, respectively. During the same interval RNA increased by 101%, while the increase in protein was much smaller, and the amount of DNA remained almost unchanged.
Doses of [2-14C]uridine, [5-3H]cytidine and [5-3H]orotic acid were administered to mice 20 min before killing. After [2-14C]uridine injection the specific activity of the uridine 5′-nucleotide fraction 20 h after the oil stimulus had not changed, while the specific activity of RNA increased by 43% during the 20-h interval. Similar results were obtained when [5-3H]-cytidine was injected. In contrast, after an injection of [5-3H]orotic acid the specific activities of both the uridine 5′-nucleotide and RNA fractions decreased with increasing time after the intraluminal injection of oil. When approximate rates of RNA synthesis were calculated independently from the data for [2-14C]uridine and [5-3H]orotic acid incorporation in these two fractions, the results agreed well, and indicated that the rate of synthesis of RNA in the decidualizing uterus increased by 64% and 163% at 8 and 20 h respectively after the intraluminal administration of sesame oil.
Journal of Endocrinology is committed to supporting researchers in demonstrating the impact of their articles published in the journal.
The two types of article metrics we measure are (i) more traditional full-text views and pdf downloads, and (ii) Altmetric data, which shows the wider impact of articles in a range of non-traditional sources, such as social media.
More information is on the Reasons to publish page.
| Sept 2018 onwards | Past Year | Past 30 Days | |
|---|---|---|---|
| Full Text Views | 1 | 0 | 0 |
| PDF Downloads | 0 | 0 | 0 |
Online ISSN: 1479-6805
Print ISSN: 0022-0795
Strengthening biomedical communities
to advance science and health
CONTACT US
Bioscientifica Ltd | Starling House | 1600 Bristol Parkway North | Bristol BS34 8YU | UK
Bioscientifica Ltd | Registered in England no 3190519
