FOETAL AND MATERNAL PLASMA PROGESTERONE CONCENTRATIONS IN THE SOW

in Journal of Endocrinology
Authors:
R. J. BARNES
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R. S. COMLINE
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MARIAN SILVER
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Intravascular catheterization of the uterine and umbilical vessels, which was first developed for use chronically in ruminants (Meschia, Cotter, Breathnach & Barron, 1965) has been used in the present acute experiments on pregnant sows (75 to 110 days' gestation). The animals were anaesthetized with sodium pentobarbitone. Vinyl catheters, filled with heparin-saline, were placed in the maternal aorta, vena cava and the uterine veins of each horn. Similar catheters were also inserted into the umbilical artery and vein through placental branches without exposure of either the cord or foetus. The Po2, Pco2 and pH of both the foetal and maternal blood were recorded during each experiment which from the induction of anaesthesia to the final sample usually lasted 5–6 h. In some animals uterine and umbilical blood flows were estimated by the diffusion—equilibrium method of Crenshaw, Huckabee, Curet, Mann & Barron (1968).

Plasma samples were separated immediately and

 

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