*Department of Surgery, University of Dundee, Dundee, DD1 4HN and †Department of Chemical Pathology, St Thomas's Hospital, London, SE1 7EH
(Received 23 August 1974)
Short-term inhibition of luteinizing hormone releasing hormone (LH-RH) in vivo can be conveniently studied by injection of antisera (Fraser & Gunn, 1973), and in the ovariectomized rat this was found to reduce both luteinizing hormone (LH) and follicle-stimulating hormone (FSH) levels when measured 7 and 24 h after injection (Koch, Chobsieng, Zor, Fridkin & Lindner, 1973). Long-term inhibition of LH-RH cannot be satisfactorily achieved by injection of antisera but is induced by active immunization. In a previous study we have shown in the male rat that this results in low levels of LH and FSH in serum and pituitary, suggesting that LH-RH is required for synthesis as well as release of both LH and FSH (Fraser, Gunn, Jeffcoate & Holland, 1974). In this study, the work
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