SPECIFICITY OF OXYTOCIN AND VASOPRESSIN IMMUNOFLUORESCENCE

in Journal of Endocrinology
Authors: D. F. SWAAB and C. W. POOL
View More View Less
Restricted access

Netherlands Central Institute for Brain Research, Ijdijk 28, Amsterdam0, The Netherlands

(Received 6 November 1974)

INTRODUCTION

Although immunohistochemistry is considered to provide a specific and sensitive tool for the localization of hypothalamic hormones (Zimmerman, Hsu, Ferin & Koslowski, 1974), attempts to localize vasopressin and oxytocin by immunofluorescence have raised questions about the specificity of this technique. Homozygous Brattleboro rats were used in our experiments as a control for vasopressin immunofluorescence since their hypothalamo-neurohypophysial system (HNS) does not contain any measurable amount of this hormone (Valtin, Sawyer & Sokol, 1965). Despite this, bright fluorescence was observed in the HNS of these animals, not only using antibodies against oxytocin, but also with all tested antibodies raised against lysine- or arginine-vasopressin. In addition, immunofluorescence was observed beyond the HNS of Wistar and heterozygous Brattleboro rats, i.e. in the suprachiasmatic nucleus.

Because of these findings and the fact that the commonly used

 

      Society for Endocrinology

Sept 2018 onwards Past Year Past 30 Days
Abstract Views 166 70 0
Full Text Views 51 2 0
PDF Downloads 63 2 0