Department of Physiology, Medical School, Birmingham, B15 2TJ and Birmingham and Midland Hospital for Women, Birmingham, BU 4HL
(Received 13 March 1975)
Because there is evidence to suggest that the level of follicle-stimulating hormone (FSH) in the hypothalamus is a factor controlling FSH secretion from the adenohypophysis (Corbin & Story, 1967; Fraschini, Motta & Martini, 1967) we have made an autoradiographic study of the hypothalamic uptake of 125I-labelled FSH in hypophysectomized adult male rats.
Human pituitary FSH was labelled with 125I by the method of Redshaw & Lynch (1974). Labelling caused a loss of less than 5% in biological potency. One microgram of the product contained 8 i.u. FSH, 0·17 i.u. luteinizing hormone and 80 μCi 125I. Human serum albumin was iodinated by the same method to the same specific activity. Six hypophysectomized albino rats each received 2 μg labelled FSH via a cannula, while labelled albumin was administered to
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