Institute of Animal Physiology and Genetics, Czechoslovak Academy of Sciences, Uhrineves, Prague, Czechoslovakia
(Received 19 March 1975)
Nowadays organ explant cultures of mammary tissue are used to study the hormonal control of mammary development in non-ruminants. We have extended this technique to the culture of goat mammary gland.
Nulliparous pure-bred Czech-white female goats with no obvious udder growth before pregnancy were used. They were killed by exsanguination and both mammary glands were removed and put in cold, sterile 0·85% NaCl. Batches of 20 expiants, selected at random, were put into Petri dishes containing 4 ml medium (Waymouth, 1959) and the required hormones. The Petri dishes were incubated at 37 °C under 95% O2 : 5% CO2. The secretory status of the expiants was studied histologically and graded according to Barnawell (1965).
The mammary tissue of our goats at weeks 9 and 10 of pregnancy contained collections of
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