EFFECTS OF PROSTAGLANDIN F AND E2 ON THE PRODUCTION OF PROGESTERONE BY HUMAN GRANULOSA CELLS IN TISSUE CULTURE

in Journal of Endocrinology
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SUMMARY

Human granulosa cells with differing steroidogenic potentials were cultured in vitro. The effects of prostaglandin F (PGF) and PGE2 on the progesterone output and viability of these cells were investigated.

Prostaglandin F either alone or in combination with LH and FSH inhibited the production of progesterone over a wide range of concentrations (1–8000 ng/ml). However, the inhibitory effect of PGF was 200 times less effective when the cells were exposed to LH and FSH for 6 days before the addition of the prostaglandin. By contrast PGE2, at concentrations from 1 to 500 ng/ml, markedly stimulated the production of progesterone by granulosa cells, and this was not prevented by the addition of PGF. The degree of inhibition by PGF or stimulation by PGE2 was related to the biosynthetic capacity of the cells.

These studies suggest that PGF may act directly on the adenylate cyclase system of human granulosa cells by blocking its activation by LH, and they demonstrate that functional regression of the luteal cell can be induced independently of the blood vascular system.

 

      Society for Endocrinology

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