FRUCTOSE: INHIBITION OF GLUCAGON AND STIMULATION OF INSULIN RESPONSES TO ARGININE IN THE ISOLATED PERFUSED RAT PANCREAS

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Third Division, Department of Medicine, University of Kobe, Kobe, Japan

(Received 21 November 1975)

It has been shown that glucagon secretion from the perfused rat pancreas is enhanced by arginine (Assan, Boillot, Attali, Soufflet & Ballerio, 1972) and suppressed by glucose (Gerich, Charles & Grodsky, 1974). However, the effect of fructose on glucagon secretion remains unclear, although fructose is known to potentiate glucose- and mannose-induced insulin secretion (Curry, Curry & Gometz, 1972; Curry, 1974). The present study investigated the effect of fructose on arginine-induced glucagon and insulin release in perfused rat pancreas.

The perfusion of isolated rat pancreas was performed by the procedure described by Grodsky, Batts, Bennett, Vcella, McWilliams & Smith (1963) with minor modifications. The splenic vessels were ligatured at the hilum, and the left gastric vessels were also ligatured. Perfusate (3·8% dextran and 0·25% bovine serum albumin in Krebs-Ringer-bicarbonate buffer) containing 2·8 mm-glucose (Expt 1),

 

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