University of Oxford, John Radcliffe Hospital, Department of Obstetrics and Gynaecology, and Nuffield Institute for Medical Research, Headley Way, Headington, Oxford, 0X3 9DU
(Received 29 December 1975)
Although steroid hormones have well recognized effects on the brain and uterus, and their specific uptake in target tissues has been demonstrated (see Baulieu, 1975), there is little information concerning the concentrations of steroids in these areas of the body. In the present study we have compared the concentration of two steroids, progesterone and oestradiol, in neuroendocrine and uterine tissues of ovariectomized sheep treated with physiological amounts of the hormones.
Sixteen sexually mature ewes (Clun Forest or Border Leicester) were bilaterally ovariectomized through a mid-ventral incision during the period of seasonal anoestrus. The animals were divided into four equal groups and received the following hormone injections (i.m., twice daily at 09.00 and 18.00 h for 10 days: Group I, 0·5 ml ethanol (vehicle
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