INDUCTION OF LACTOSE SYNTHESIS IN THE MAMMARY GLANDS OF PREGNANT RATS BY OVARIECTOMY

in Journal of Endocrinology
Authors:
J. SHANI
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K. OTA
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H. TOMOGANE
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A. YOKOYAMA
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Faculty of Agriculture, Nagoya University, Nagoya 464, Japan

(Received 6 July 1976)

In 1965, Shinde, Ôta & Yokoyama reported that the initiation of lactose synthesis in the mammary gland could be advanced by ovariectomy and/or foeto-placentectomy performed on days 18 or 19 of pregnancy in the rat. The present experiments were designed to determine the stage of pregnancy at which the mammary gland was able to initiate lactose synthesis in response to ovariectomy. Corticosterone in plasma was also measured in relation to the changes around the time of lactogenesis reported previously (Ôta, Ôta & Yokoyama, 1974).

Primigravid Wistar-Imamichi strain rats, weighing between 200 and 280 g, were kept in a temperature- (23 ± 2°C) and light- (14h light: 10h darkness, lights on 05.00h) controlled animal room. The day on which sperm in the vagina or vaginal plugs were found was designated day 0 of pregnancy. Ovariectomy was performed between 17.30 and 18.00

 

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