Laboratoire de Physiologie de la Reproduction, CNRS, Hôpital de Bicêtre (INSERM), 94270 Bicêtre, France
(Received 4 January 1977)
Each endometrial population of cells responds differently to ovarian hormones in vivo (Tachi, Tachi & Lindner, 1972; Finn & Porter, 1975). For in-vitro studies on the effects of hormones on individual cell populations, the separation of the luminal epithelium from the stromal tissue presents a major difficulty (Smith, Martin, King & Vertes, 1970; Gerschenson, Berliner & Yang, 1974; Boshier & Katz, 1975; Heald, Govan & O'Grady, 1975). In this report a simple and rapid method for separating the uterine epithelium of the rat is described with details of its use for primary cell culture. Such cultures when carried out in scintillation vials or in depression slides are particularly suitable for studies involving incorporation of radioactive isotopes.
The uterine horns were removed, separated and cleaned free of fat and connective tissues. They were
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