AUGMENTATION OF VASOPRESSIN ANTIDIURESIS BY PROLACTIN WITH A NOTE ON THE CONTAMINATION OF INTERNATIONAL STANDARD PROLACTIN

in Journal of Endocrinology
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A. K. WATERS
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Department of Physiology, Charing Cross Hospital Medical School, London, W6

(Received 7 April 1977)

It has been reported (Horrobin, Burstyn, Lloyd, Durkin, Lipton & Muiruri, 1971; Buckman & Peake, 1973; Wallin & Lee, 1976) that prolactin affects salt and water metabolism. To assess any interaction of the antidiuretic hormone arginine-vasopressin (AVP) and prolactin, four experiments were performed.

The antidiuretic action of AVP was measured by the standard AVP bioassay method using the water-loaded ethanol-anaesthetized rat described by Jeffers, Livesey & Austin (1942) with the minor modifications of Forsling, Jones & Lee (1968). Male Wistar rats (150-200 g) were used with at least six preparations for each experiment. When stable antidiuretic responses to standard AVP (3rd International Standard, 1957, National Institute for Biological Standards, London) were obtained, the infusion fluid was changed to one containing the appropriate hormone for the experiment and the effect of AVP within the succeeding hour compared

 

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