MRC Blood Pressure Unit, Western Infirmary, Glasgow, Gil 6NT
(Received 19 December 1977)
Only a few of the radioimmunoassays for vasopressin (AVP) published previously (Dunn, Brennan, Nelson & Robertson, 1973; Möhring & Möhring, 1975) have been sensitive enough for use in the rat. This communication describes a novel method for extraction of AVP from plasma involving the use of a solid-phase antibody and its application in a radioimmunoassay sufficiently sensitive to detect this hormone in rat plasma.
Antiserum to AVP (102/5, 250 μl; Morton, Padfield & Forsling, 1975) was coupled to CNBr-Sepharose 4B (2 g) by mixing for 18 h at room temperature in 0·1 m-NaHC03-0·5 m-NaCl (15–20 ml). The solid antiserum was mixed with 0·2 m-Tris-HCl, pH 8·0, for 18 h and then washed with 1 m-acetic acid (200 ml). The solid antibody was finally equilibrated with 42·5 ml 0-05 m-Tris-HCl, pH 7·5, to give an antiserum dilution of
Journal of Endocrinology is committed to supporting researchers in demonstrating the impact of their articles published in the journal.
The two types of article metrics we measure are (i) more traditional full-text views and pdf downloads, and (ii) Altmetric data, which shows the wider impact of articles in a range of non-traditional sources, such as social media.
More information is on the Reasons to publish page.
| Sept 2018 onwards | Past Year | Past 30 Days | |
|---|---|---|---|
| Full Text Views | 2 | 0 | 0 |
| PDF Downloads | 2 | 0 | 0 |
Online ISSN: 1479-6805
Print ISSN: 0022-0795
Strengthening biomedical communities
to advance science and health
CONTACT US
Bioscientifica Ltd | Starling House | 1600 Bristol Parkway North | Bristol BS34 8YU | UK
Bioscientifica Ltd | Registered in England no 3190519
