Department of Physiology and * Division of Nephrology, University of Maryland School of Medicine, Baltimore, Maryland 21201, U.S.A. and †The United States Department of Agriculture, Beltsville, Maryland 20705, U.S.A.
(Received 26 June 1978)
The chemical composition of human ovarian follicular fluid has been well characterized (Shalgi, Kraicer & Soferman, 1972; Edwards, 1974). However, compositional studies of porcine follicular fluid (PFF; Schuetz, 1974; Chang, Jones, Ellefson & Ryan, 1976), have not included acid–base measurements (pH, oxygen and carbon dioxide tension (Po2, Pco2), concentration of bicarbonate HCO3−]). Recent studies of the nuclear maturation of porcine oocytes have demonstrated a rate of maturation of only 55–80% for oocytes collected from ovaries obtained at the abattoir (Tsafriri & Channing, 1975) and it was suggested that maturing and non-maturing oocytes may have undergone nuclear degeneration. It has also been shown in monkeys that oocytes which appear mature when examined by light microscopy may in fact
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