The present studies examined the inhibitory effect of mid-cycle and preovulatory bovine follicular fluid (bFF) on the secretion of progesterone and prostaglandin F2α (PGF2α) by bovine theca and granulosa cells cultured in tissue culture Medium 199. The inhibitory effect of bFF on the secretion of PGF2α by incubated and cultured hemipituitary glands of male rats was also studied. Addition of 5, 10 or 20% charcoal-extracted, mid-cycle bFF to the culture medium caused a twofold decrease in the accumulation of both progesterone and PGF2α by the cultured granulosa cells (P < 0·01). In contrast, when preovulatory bFF was used, there was no inhibitory effect on secretion of progesterone and PGF2α. The addition of 10% mid-cycle bFF to the culture medium caused a two- to fourfold decrease in the accumulation of both PGF2α and progesterone by the cultured theca (P < 0·008). However, in the presence of 1 μg LH/ml, the inhibitory effect of mid-cycle bFF on progesterone and PGF2α secretion was abolished. The secretion of PGF2α was significantly (P < 0·03) decreased in male rat hemipituitary glands after 5 h of incubation or 18 h of culture. These findings suggest that bFF from mid-cycle follicles inhibits prostaglandin synthetase as well as luteinization. The inhibition disappeared with bFF from preovulatory follicles.
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Online ISSN: 1479-6805
Print ISSN: 0022-0795
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