Oxytocin was measured radioimmunologically during the oestrous cycle in six ewes of each of two local Moroccan breeds of sheep. Concentrations in both breeds approached the lower limit of the assay (3 pg/ml) from 2 days before oestrus, throughout heat and ovulation until day 2 of the cycle. Oxytocin concentrations then increased in both breeds, the resulting highest levels on days 5–7 were, on average, between 30 and 60 pg/ml in the D'man sheep and 13–31 pg/ml in the Timhadite breed. Oxytocin levels then decreased to about 7 pg/ml in the D'man and about 4–5 pg/ml in the Timhadite breed on days 14–15. After ovariectomy oxytocin concentrations remained at about the limit of detection for a further 19–20 days in both breeds.
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