Measurement of immunoreactive α-melanocyte-stimulating hormone in the blood of rainbow trout kept under various conditions

in Journal of Endocrinology
Authors:
T. J. Bowley
Search for other papers by T. J. Bowley in
Current site
Google Scholar
PubMed
Close
,
T. A. Rance
Search for other papers by T. A. Rance in
Current site
Google Scholar
PubMed
Close
, and
B. I. Baker
Search for other papers by B. I. Baker in
Current site
Google Scholar
PubMed
Close
Restricted access
Rent on DeepDyve

Sign up for journal news

Initial attempts to measure α-MSH in trout blood by radioimmunoassay proved unsuccessful due to apparent losses of immunoreactive hormone, especially in the plasma of trout adapted to a black background. An extraction protocol for α-MSH from plasma is described. Subsequent radioimmunoassay revealed progressive increases in immunoreactive α-MSH as trout became adapted to a black background. Plasma α-MSH titres were raised during fungal infection, and also showed significant differences in fish obtained from different fish farms.

 

  • Collapse
  • Expand