Inactivation of prostaglandin E2 by rat isolated lung during the oestrous cycle

in Journal of Endocrinology
Authors:
Y. S. Bakhle
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J. T. Zakrzewski
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The metabolism of prostaglandin E2 (PGE2) in lungs from female rats was measured during the stages of the oestrous cycle. In isolated lungs perfused through the pulmonary circulation, only 7–20% of PGE2 escaped metabolism, as measured by bioassay and radioimmunoassay. Within these limits, survival was highest at pro-oestrus compared with metoestrus and dioestrus. Uptake of PGE2 from the pulmonary vasculature, assessed by measuring the efflux of radioactivity derived from [14C]PGE2 injected into the pulmonary circulation of the isolated lung, did not show cycle-related variations. Assay of [14C]PGE2 metabolism by tissue homogenates prepared from lungs taken at different stages of the oestrous cycle showed a significant decrease in enzyme activity at pro-oestrus compared with dioestrus. It is concluded that PGE2 metabolism in isolated rat lung is affected by the oestrous cycle and that the increased PGE2 survival at pro-oestrus may be more readily explained by changes in enzyme activity than by changes in uptake of substrate.

 

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