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Laurie K Bale and Cheryl A Conover

Introduction Insulin-like growth factor (IGF)-II is a major determinant of fetal growth. Targeted disruption of the mouse IgfII gene produces proportional dwarfs, which are 60% of normal birthweight ( DeChiara et al. 1990 ). The

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Rumana Yasmeen, Qiwen Shen, Aejin Lee, Jacob H Leung, Devan Kowdley, David J DiSilvestro, Lu Xu, Kefeng Yang, Andrei Maiseyeu, Naresh C Bal, Muthu Periasamy, Paolo Fadda, and Ouliana Ziouzenkova

, Elk Grove Village, IL, USA). Gene expression quantification by NanoString nCounter assay NanoString’s nCounter (NanoString Technologies) analysis system performs direct detection of target molecules without an amplification step. Target

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Wenpeng Dong, Ye Jia, Xiuxia Liu, Huan Zhang, Tie Li, Wenlin Huang, Xudong Chen, Fuchun Wang, Weixia Sun, and Hao Wu

2-related factor 2 (NRF2) has been identified as a governor of antioxidant and redox signaling. NRF2 activates the transcription of downstream antioxidant genes such as heme oxygenase-1 ( Ho1 ) and NAD(P)H dehydrogenase quinone 1 ( Nqo1 ) ( Ruiz et

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Eric W-F Lam, Kunal Shah, and Jan J Brosens

pri-miRNAs and, consequently, the mRNA targets of these miRNAs were more abundantly expressed ( Yamagata et al . 2009 ). Figure 1 Biogenesis of miRNAs. Micro-RNA genes are transcribed from the genome by RNA polymerase II to generate primary

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Malin Fex, Nils Wierup, Marloes Dekker Nitert, Michael Ristow, and Hindrik Mulder

Introduction Gene inactivation, using the Cre/LoxP system, is an essential technique in tissue-specific gene targeting. The rat insulin promoter 2-Cre recombinase (RIP2-Cre) mouse (also called RIP-Cre or B6.Cg-Tg (Ins2-cre) 25 Mgn

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Ljupka Gligorovska, Biljana Bursać, Sanja Kovačević, Nataša Veličković, Gordana Matić, and Ana Djordjevic

-enriched diet. Glucose metabolism was assessed through analysis of systemic insulin sensitivity, while lipid metabolism was analyzed at the level of visceral adiposity, VAT histology and the expression of GC-target genes involved in lipogenesis. Besides GR

Open access

Andrea Mafficini and Aldo Scarpa

this milestone, especially with regard to targeted therapy ( Capdevila et al . 2017 , Gajate et al . 2017 ). Table 1 Genes implicated in the tumorigenesis of pancreatic neuroendocrine tumours (PanNETs) before the publication of the

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Ya Liu, Xiaoqing Zhou, Ye Xiao, Changjun Li, Yan Huang, Qi Guo, Tian Su, Lei Fu, and Liping Luo

oligonucleotide primer pairs used in qRT-PCR analysis. Target gene Sense primer Antisense primer Chrebp 5′-CCTCACTTCACTGTGCCTCA-3′ 5′-ACAGGGGTTGTTGTCTCTGG-3′ Srebp1c 5′-GGAGCCATGGATTGCACATT-3′ 5′-GGAAGTCACTGTCTTGGTTGTTGA-3

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Inga K Johnsen, Marc Slawik, Igor Shapiro, Michaela F Hartmann, Stefan A Wudy, Brendan D Looyenga, Gary D Hammer, Martin Reincke, and Felix Beuschlein

, numerous transcriptional cascades are utilized to ensure tight control over cellular proliferation and proper spatio-temporal expression of target genes. A gonadal specific target gene which is expressed in adrenal tumors of CE/J mice is the Mullerian

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Vicki E Smith, Jayne A Franklyn, and Christopher J McCabe

a down-stream target for pituitary tumor-transforming gene . Journal of Biological Chemistry 276 8484 – 8491 doi:10.1074/jbc.M009654200 . Reynolds LE Watson AR Baker M Jones TA D'Amico G Robinson SD Joffre C Garrido-Urbani S